Journal: bioRxiv

Article Title: Early life γδ T cell activation enforces intestinal barrier integrity during intergenerational C. difficile colonization

doi: 10.64898/2026.04.28.721483

Figure Lengend Snippet: The early life IL-17 response is dependent on IL-1 β produced by infiltrating myeloid cells. (A) Differential abundance using MiloR analysis of single cell sequencing of total CD45+ cells of weanlings and adults. (B, C) Total number of neutrophils(B) and monocytes (C) in the colon normalized to weight of the tissue of mice at indicated ages. (D, E) Total number of neutrophils (D) and monocytes (E) in the colon lamina propria of 3-week old littermate IL-17+/-and IL17-/-mice. (F) UMAP of Il1b expression in weanling and adult colon CD45+ cells. (G) Expression of Il1b in total colon of mice at indicated ages. (H, I) Representative flow plot (H) and percent (I) of pro-IL-1β+ myeloid cells in the LP of weanling and adult colons. (J) ELISA quantification of IL-1β in the supernatant of colon explants of weanling and adult mice, normalized to weight of tissue. (K, L) Percent of IL-17+ γδ T cells (K) and percent of neutrophils (L) in colonic LP of control mice and mice treated with anti-IL-1R in vivo antibody from 2-3 weeks. (M, N) Percent of IL-17+ γδ T cells (M) and percent of neutrophils (N) in colonic LP of littermate IL-1R+/-and IL-1R-/-at 3 weeks of age. (O) UMAP of type 3 immunity score and Il1r1 expression on total thymocytes from day 0 mouse (P) Percent of IL-17+ γδ T cells in 3-week-old TCRδ-/-adoptively transferred with IL-1R+/-or IL-1R-/-thymus cells. (Q) UMAP of IL-1R1 expression in total CD45+ cells in human fetal thymus γδ T cells. (R, S ) Percent of IFNγ (R) and IL-17 (S) of Vγ9+ γδ T cells in human cord blood following stimulation with IL-1β and zoledronate. (B, C, D, E, G, I-N, P, R, and S) Each dot represents cells from an individual mouse. Data are represented as mean with SD. Graphs are pooled data from at least two independent experiments with atleast n = 3 in each experiment. (H) Contour plots are representative at least two independent experiments. Numbers indicate frequency of cells within each quadrant or box. (B, C, G) Data were analyzed using Kruskal-Wallis ANOVA test or (D, E, I-N and P) Mann-Whitney non-parametric unpaired T test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p <0.0001).

Article Snippet: IL-1r1-/-mice (C57BL/6, No.003245) were obtained from Jackson laboratories.

Techniques: Produced, Single Cell, Sequencing, Expressing, Enzyme-linked Immunosorbent Assay, Control, In Vivo, MANN-WHITNEY

Journal: Journal of Molecular Medicine (Berlin, Germany)

Article Title: Cell-specific conditional deletion of interleukin-1 (IL-1) ligands and its receptors: a new toolbox to study the role of IL-1 in health and disease

doi: 10.1007/s00109-020-01928-5

Figure Lengend Snippet: Generation of IL-1α fl/fl , IL-1β fl/fl , and IL-1R1 fl/fl mice. A Exon 4 of the Il1a gene (for IL-1α fl/fl ), B exon 4–5 of the Il1b gene (for IL-1β fl/fl mice), or C exon 5 of the Il1r1 gene (for IL-1R1 fl/fl ) flanked with loxP sites is excised upon Cre recombination, resulting in cell-specific IL-1α-, IL-1β-, or IL-1R1-deficient allele, respectively

Article Snippet: Further, IL-1R1 −/− mice, originally generated by Immunex by targeted deletion of exon 1 and 2 of the Il1r1 gene [ ], showed that most, but not all, IL-1 actions are mediated by IL-1R1 (see [ ] for review).

Techniques: